Sabbadin, Federico, Henrissat, Bernard, Bruce, Neil Charles orcid.org/0000-0003-0398-2997 et al. (1 more author) (2021) Lytic Polysaccharide Monooxygenases as Chitin-Specific Virulence Factors in Crayfish Plague. Biomolecules. v.11(8) 1180. ISSN 2218-273X
Abstract
he oomycete pathogen Aphanomyces astaci, also known as "crayfish plague", is an obligate fungal-like parasite of freshwater crustaceans and is considered responsible for the ongoing decline of native European crayfish populations. A. astaci is thought to secrete a wide array of effectors and enzymes that facilitate infection, however their molecular mechanisms have been poorly characterized. Here, we report the identification of AA15 lytic polysaccharide monooxygenases (LPMOs) as a new group of secreted virulence factors in A. astaci. We show that this enzyme family has greatly expanded in A. astaci compared to all other oomycetes, and that it may facilitate infection through oxidative degradation of crystalline chitin, the most abundant polysaccharide found in the crustacean exoskeleton. These findings reveal new roles for LPMOs in animal-pathogen interactions, and could help inform future strategies for the protection of farmed and endangered species.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021, The Author(s). |
Keywords: | crayfish plague, oomycete, lytic polysaccharide monooxygenase, chitin, pathogen, infection, virulence |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) > Centre for Novel Agricultural Products (CNAP) (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Funding Information: | Funder Grant number BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/T001496/1 BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/L001926/1 |
Depositing User: | Pure (York) |
Date Deposited: | 03 Sep 2021 11:10 |
Last Modified: | 26 Nov 2024 00:51 |
Published Version: | https://doi.org/10.3390/biom11081180 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.3390/biom11081180 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:177821 |