Hoffmann, S, Fuenzalida Werner, JP, Moreno-Villoslada, I et al. (1 more author) (2016) New insights into the nature of the Cibacron brilliant red 3B-A – Chitosan interaction. In: Pure and Applied Chemistry. 12th Conference of the European Chitin Society (12th EUCHIS)/13th International Conference on Chitin and Chitosan (13th ICCC), 30 Aug - 02 Sep 2015, Münster, Germany. De Gruyter , pp. 891-904.
Abstract
Cibacron brilliant red 3B-A (CBR) has been introduced to determine chitosan (CS) concentrations in solution, and several studies applied it to measure chitosan content in pharmaceutical formulations. So far, studies have relied on the absorbance band shift to 570 nm to determine the extent of the CBR – CS interaction. In this study, we show that CBR forms micro- to nanometer sized aggregates with CS, depending on their charge ratio and that other photophysical changes in CBR are induced by this interaction. We found that, besides the bathochromic band shift, aggregation induces emission at 600 nm and emission quenching at 360 nm. We compared changes CS induced in absorbance and fluorescence emission of CBR with the CS monomer glucosamine and poly(allylamine) hydrochloride, which both contain amino groups, and found that similar but less intense photophysical changes also occur. Furthermore, CS-induced circular dichroism in CBR suggests a twisted, chiral structure of these aggregates that should match with the previously published in silico simulations of the structure of CS in solution. The low linear charge density of CS and its chiral conformation are considered responsible for the enhanced photophysical response of CBR interacting with the polycation.
Metadata
Item Type: | Proceedings Paper |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | ©2016 IUPAC & De Gruyter. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. For more information, please visit: http://creativecommons.org/licenses/by-nc-nd/4.0/. |
Keywords: | aggregation; chitosan; dye interaction; EUCHIS-12; ICCC-13; polyelectrolyte–dye interactions; polyelectrolytes |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Chemistry and Biochemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 23 Aug 2021 09:05 |
Last Modified: | 23 Aug 2021 09:05 |
Status: | Published |
Publisher: | De Gruyter |
Identification Number: | 10.1515/pac-2016-0712 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:177306 |