Tomlinson, MJ, Tomlinson, S, Yang, XB orcid.org/0000-0002-0144-2826 et al. (1 more author) (2013) Cell separation: Terminology and practical considerations. Journal of Tissue Engineering, 4 (1). pp. 1-14. ISSN 2041-7314
Abstract
Cell separation is a powerful tool in biological research. Increasing usage, particularly within the tissue engineering and regenerative medicine communities, means that researchers from a diverse range of backgrounds are utilising cell separation technologies. This review aims to offer potential solutions to cell sorting problems and to clarify common ambiguities in terminology and experimental design. The frequently used cell separation terms of 'purity', 'recovery' and 'viability' are discussed, and attempts are made to reach a consensus view of their sometimes ambiguous meanings. The importance of appropriate experimental design is considered, with aspects such as marker expression, tissue isolation and original cell population analysis discussed. Finally, specific technical issues such as cell clustering, dead cell removal and non-specific antibody binding are considered and potential solutions offered. The solutions offered may provide a starting point to improve the quality of cell separations achieved by both the novice and experienced researcher alike.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Keywords: | cell separation; cell sorting; terminology; cell clusters; dead cells; tissue preparation |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 Aug 2021 10:46 |
Last Modified: | 13 Aug 2021 10:46 |
Status: | Published |
Publisher: | SAGE Publications |
Identification Number: | 10.1177/2041731412472690 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:176958 |