Relish plays a dynamic role in the niche to modulate drosophila blood progenitor homeostasis in development and infection

Abstract

Immune challenges demand the gearing up of basal hematopoiesis to combat infection. Little is known about how during development, this switch is achieved to take care of the insult. Here, we show that the hematopoietic niche of the larval lymph gland of Drosophila senses immune challenge and reacts to it quickly through the nuclear factor-κB (NF-κB), Relish, a component of the immune deficiency (Imd) pathway. During development, Relish is triggered by ecdysone signaling in the hematopoietic niche to maintain the blood progenitors. Loss of Relish causes an alteration in the cytoskeletal architecture of the niche cells in a Jun Kinase dependent manner, resulting in the trapping of Hh implicated in progenitor maintenance. Notably, during infection, downregulation of Relish in the niche tilts the maintenance program towards precocious differentiation, thereby bolstering the cellular arm of the immune response.

Metadata

Item Type:	Article
Authors/Creators:	Ramesh, Parvathy Dey, Nidhi Sharma (nidhi.dey@york.ac.uk) Kanwal, Aditya Mandal, Sudip Mandal, Lolitika
Copyright, Publisher and Additional Information:	Funding Information: We thank B Lemaitre, N Silverman, I Ando, U Banerjee, S Cohen, P Ingham and M Crozatier A. D. Shirras, R. Schulz and N.G. Prasad for reagents. Special thanks to Sushmit Ghosh and Kaustuv Ghosh for assisting with FISH and germ-free experiments. We thank all members of the two laboratories for their valuable inputs. We thank USER Mohali?s Confocal Facility, Bloomington Drosophila Stock Center, at Indiana University, VDRC (Vienna) and Developmental Studies Hybridoma Bank, University of Iowa for flies and antibodies. Models ?Created with BioRender.com?. DBT Wellcome-Trust India Alliance Senior Fellowship [IA/S/17/1/503100] to LM and Institutional support to PR, NSD, SM and DBT-fellowship funding to AK for this study duly acknowledged. Publisher Copyright: © 2021, eLife Sciences Publications Ltd. All rights reserved.
Dates:	Published: 22 July 2021 Accepted: 22 July 2021
Institution:	The University of York
Academic Units:	The University of York > Faculty of Sciences (York) > Hull York Medical School (York)
Depositing User:	Pure (York)
Date Deposited:	27 Jul 2021 10:30
Last Modified:	21 Jan 2025 17:55
Published Version:	https://doi.org/10.7554/eLife.67158
Status:	Published
Refereed:	Yes
Identification Number:	10.7554/eLife.67158
Related URLs:	http://www.scopus.com/inward/record.url?...
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:176558