Bury, AG, Vincent, AE, Turnbull, DM et al. (2 more authors) (2020) Mitochondrial isolation: when size matters. Wellcome Open Research, 5. 226. ISSN 2398-502X
Abstract
Mitochondrial vitality is critical to cellular function, with mitochondrial dysfunction linked to a growing number of human diseases. Tissue and cellular heterogeneity, in terms of genetics, dynamics and function means that increasingly mitochondrial research is conducted at the single cell level. Whilst there are several technologies that are currently available for single-cell analysis, each with their advantages, they cannot be easily adapted to study mitochondria with subcellular resolution. Here we review the current techniques and strategies for mitochondrial isolation, critically discussing each technology’s limitations for future mitochondrial research. Finally, we highlight and discuss the recent breakthroughs in sub-cellular isolation techniques, with a particular focus on nanotechnologies that enable the isolation of mitochondria from subcellular compartments. This allows isolation of mitochondria with unprecedented spatial precision with minimal disruption to mitochondria and their immediate cellular environment.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 Bury AG et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Mitochondria, mtDNA, mitochondrial isolation, heterogeneity, subcellular, nanoprobes, nanobiopsy, nanotweezers |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 28 Jul 2021 14:50 |
Last Modified: | 28 Jul 2021 14:50 |
Status: | Published |
Publisher: | F1000 Research |
Identification Number: | 10.12688/wellcomeopenres.16300.2 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:176373 |