Findlay, Rachel C, Osman, Mohamed, Spence, Kirstin A et al. (3 more authors) (2021) High-speed, three-dimensional imaging reveals chemotactic behaviour specific to human-infective Leishmania parasites. eLife. e65051. ISSN 2050-084X
Abstract
Cellular motility is an ancient eukaryotic trait, ubiquitous across phyla with roles in predator avoidance, resource access, and competition. Flagellar motility is seen in various parasitic protozoans, and morphological changes in flagella during the parasite life cycle have been observed. We studied the impact of these changes on motility across life cycle stages, and how such changes might serve to facilitate human infection. We used holographic microscopy to image swimming cells of different Leishmania mexicana life cycle stages in three dimensions. We find that the human-infective (metacyclic promastigote) forms display 'run and tumble' behaviour in the absence of stimulus, reminiscent of bacterial motion, and that they specifically modify swimming direction and speed to target host immune cells in response to a macrophage-derived stimulus. Non-infective (procyclic promastigote) cells swim more slowly, along meandering helical paths. These findings demonstrate adaptation of swimming phenotype and chemotaxis towards human cells.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021, Findlay et al. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Hull York Medical School (York) The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Physics (York) |
Depositing User: | Pure (York) |
Date Deposited: | 29 Jun 2021 11:40 |
Last Modified: | 10 Dec 2024 00:19 |
Published Version: | https://doi.org/10.7554/eLife.65051 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.7554/eLife.65051 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:175718 |