Oedekoven, Caroline A, Belmonte, Miriam, Bode, Daniel et al. (12 more authors) (2021) Hematopoietic stem cells retain functional potential and molecular identity in hibernation cultures. Stem Cell Reports. pp. 1614-1628. ISSN 2213-6711
Abstract
Advances in the isolation and gene expression profiling of single hematopoietic stem cells (HSCs) have permitted in-depth resolution of their molecular program. However, long-term HSCs can only be isolated to near purity from adult mouse bone marrow, thereby precluding studies of their molecular program in different physiological states. Here, we describe a powerful 7-day HSC hibernation culture system that maintains HSCs as single cells in the absence of a physical niche. Single hibernating HSCs retain full functional potential compared with freshly isolated HSCs with respect to colony-forming capacity and transplantation into primary and secondary recipients. Comparison of hibernating HSC molecular profiles to their freshly isolated counterparts showed a striking degree of molecular similarity, further resolving the core molecular machinery of HSC self-renewal while also identifying key factors that are potentially dispensable for HSC function, including members of the AP1 complex (Jun, Fos, and Ncor2), Sult1a1 and Cish. Finally, we provide evidence that hibernating mouse HSCs can be transduced without compromising their self-renewal activity and demonstrate the applicability of hibernation cultures to human HSCs.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 The Authors |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 08 Apr 2021 15:10 |
Last Modified: | 27 Nov 2024 00:40 |
Published Version: | https://doi.org/10.1016/j.stemcr.2021.04.002 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.stemcr.2021.04.002 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:172953 |
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Description: Hematopoietic stem cells retain functional potential and molecular identity inhibernation cultures
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Filename: PIIS2213671121001971.pdf
Description: Hematopoietic stem cells retain functional potential and molecular identity in hibernation cultures
Licence: CC-BY 2.5