Moroz, Olga V., Blagova, Elena, Taylor, Edward et al. (12 more authors) (2021) Fungal GH25 muramidases:New family members with applications in animal nutrition and a crystal structure at 0.78Å resolution. PLoS ONE. e0248190. ISSN 1932-6203
Abstract
Muramidases/lysozymes hydrolyse the peptidoglycan component of the bacterial cell wall. They are found in many of the glycoside hydrolase (GH) families. Family GH25 contains muramidases/lysozymes, known as CH type lysozymes, as they were initially discovered in the Chalaropsis species of fungus. The characterized enzymes from GH25 exhibit both β-1,4-N-acetyl- and β-1,4-N,6-O-diacetylmuramidase activities, cleaving the β-1,4-glycosidic bond between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) moieties in the carbohydrate backbone of bacterial peptidoglycan. Here, a set of fungal GH25 muramidases were identified from a sequence search, cloned and expressed and screened for their ability to digest bacterial peptidoglycan, to be used in a commercial application in chicken feed. The screen identified the enzyme from Acremonium alcalophilum JCM 736 as a suitable candidate for this purpose and its relevant biochemical and biophysical and properties are described. We report the crystal structure of the A. alcalophilum enzyme at atomic, 0.78 Å resolution, together with that of its homologue from Trichobolus zukalii at 1.4 Å, and compare these with the structures of homologues. GH25 enzymes offer a new solution in animal feed applications such as for processing bacterial debris in the animal gut.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 Moroz et al. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 26 Mar 2021 16:10 |
Last Modified: | 22 Oct 2024 00:01 |
Published Version: | https://doi.org/10.1371/journal.pone.0248190 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1371/journal.pone.0248190 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:172616 |
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Filename: journal.pone.0248190.pdf
Description: Fungal GH25 muramidases: New family members with applications in animal nutrition and a crystal structure at 0.78Å resolution
Licence: CC-BY 2.5