Cheung, K.Y. orcid.org/0000-0002-1098-4926, Jesuthasan, S.J., Baxendale, S. orcid.org/0000-0002-6760-9457 et al. (4 more authors) (2021) Olfactory rod cells : a rare cell type in the larval zebrafish olfactory epithelium with a large actin-rich apical projection. Frontiers in Physiology, 12 (1). 626080.
Abstract
We report the presence of a rare cell type, the olfactory rod cell, in the developing zebrafish olfactory epithelium. These cells each bear a single actin-rich rod-like apical projection extending 5–10 μm from the epithelial surface. Live imaging with a ubiquitous Lifeact-RFP label indicates that the olfactory rods can oscillate. Olfactory rods arise within a few hours of the olfactory pit opening, increase in numbers and size during larval stages, and can develop in the absence of olfactory cilia. Olfactory rod cells differ in morphology from the known classes of olfactory sensory neuron, but express reporters driven by neuronal promoters. A sub-population of olfactory rod cells expresses a Lifeact-mRFPruby transgene driven by the sox10 promoter. Mosaic expression of this transgene reveals that olfactory rod cells have rounded cell bodies located apically in the olfactory epithelium and have no detectable axon. We offer speculation on the possible function of these cells in the Discussion.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: | olfactory rod cell; olfactory placode; olfactory epithelium; actin; actin-rich projection; Lifeact; zebrafish |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Funding Information: | Funder Grant number Biotechnology and Biological Sciences Research Council BB/M012522/1; BB/S007008/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 18 Mar 2021 12:19 |
Last Modified: | 18 Mar 2021 12:19 |
Status: | Published |
Publisher: | Frontiers Media SA |
Refereed: | Yes |
Identification Number: | 10.3389/fphys.2021.626080 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:172096 |