Fang, X., Peden, A.A. orcid.org/0000-0003-0144-7712, van Eeden, F.J.M. et al. (1 more author) (2021) Identification of additional outer segment targeting signals in zebrafish rod opsin. Journal of Cell Science, 134 (6). jcs.254995. ISSN 0021-9533
Abstract
In vertebrate photoreceptors, opsins are highly concentrated in a morphologically distinct ciliary compartment known as the outer segment (OS). Opsin is synthesized in the cell body and transported to the OS at a remarkable rate of 100-1000 molecules per second. Opsin transport defects contribute to photoreceptor loss and blindness in human ciliopathies. Previous studies revealed that the opsin C-terminal tail, of 44 amino acids, is sufficient to mediate OS targeting in Xenopus photoreceptors. Here we show that although the Xenopus C-terminus retains this function in zebrafish, the homologous zebrafish sequence is not sufficient to target opsin to the OS. This functional difference is largely caused by a change of a single amino acid present in Xenopus, but not in other vertebrates examined. Furthermore, we find that sequences in the 3rd intracellular cytoplasmic loop (IC3) and adjacent regions of transmembrane helixes 6 and 7 are also necessary for opsin transport in zebrafish. Combined with the cytoplasmic tail, these sequences are sufficient to target opsin to the ciliary compartment.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021. Published by The Company of Biologists Ltd. This is an author-produced version of a paper subsequently published in Journal of Cell Science. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Opsin; Cilia; GPCR; Photoreceptor; Vision |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 24 Feb 2021 13:44 |
Last Modified: | 17 Feb 2022 09:58 |
Status: | Published |
Publisher: | The Company of Biologists |
Refereed: | Yes |
Identification Number: | 10.1242/jcs.254995 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:171499 |