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Galloway, J orcid.org/0000-0003-3998-0870, Bray, HEV, Shoemark, DK orcid.org/0000-0002-1240-8463 et al. (11 more authors) (2021) De Novo Designed Peptide and Protein Hairpins Self‐Assemble into Sheets and Nanoparticles. Small, 17 (10). 2100472. ISSN 1613-6810
Abstract
The design and assembly of peptide‐based materials has advanced considerably, leading to a variety of fibrous, sheet, and nanoparticle structures. A remaining challenge is to account for and control different possible supramolecular outcomes accessible to the same or similar peptide building blocks. Here a de novo peptide system is presented that forms nanoparticles or sheets depending on the strategic placement of a “disulfide pin” between two elements of secondary structure that drive self‐assembly. Specifically, homodimerizing and homotrimerizing de novo coiled‐coil α‐helices are joined with a flexible linker to generate a series of linear peptides. The helices are pinned back‐to‐back, constraining them as hairpins by a disulfide bond placed either proximal or distal to the linker. Computational modeling indicates, and advanced microscopy shows, that the proximally pinned hairpins self‐assemble into nanoparticles, whereas the distally pinned constructs form sheets. These peptides can be made synthetically or recombinantly to allow both chemical modifications and the introduction of whole protein cargoes as required.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 The Authors. Small published by Wiley‐VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) |
Keywords: | coiled coil; computational modeling; peptide design; protein design; self-assembly |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Discovery & Translational Science Dept (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 18 Feb 2021 12:54 |
Last Modified: | 08 Jan 2025 14:28 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/smll.202100472 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:171326 |
Available Versions of this Item
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De Novo Designed Peptide and Protein Hairpins that Self-assemble into Sheets and Nanoparticles. (deposited 22 Apr 2024 12:24)
- De Novo Designed Peptide and Protein Hairpins Self‐Assemble into Sheets and Nanoparticles. (deposited 18 Feb 2021 12:54) [Currently Displayed]