Santos, Á.D., Fili, N., Pearson, D.S. et al. (2 more authors) (2021) High throughput mechanobiology : force modulation of ensemble biochemical and cell-based assays. Biophysical Journal, 120 (4). pp. 631-641. ISSN 0006-3495
Abstract
Mechanobiology is focused on how the physical forces and mechanical properties of proteins, cells and tissues contribute to physiology and disease. While the response of proteins and cells to mechanical stimuli is critical for function, the tools to probe these activities are typically restricted to single molecule manipulations. Here, we have developed a novel microplate reader assay to encompass mechanical measurements with ensemble biochemical and cellular assays, using a microplate lid modified with magnets. This configuration enables multiple static magnetic tweezers to function simultaneously across the microplate, thereby greatly increasing throughput. We demonstrate the broad applicability and versatility through in vitro and in cellulo approaches. Overall, our methodology allows, for the first-time, ensemble biochemical and cell-based assays to be performed under force, in high throughput format. This approach substantially increases the availability of mechanobiology measurements.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 Biophysical Society. This is an author produced version of a paper subsequently published in Biophysical Journal. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
Keywords: | mechanobiology; tweezers; high-throughput; helicase; myosin |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) |
Funding Information: | Funder Grant number Medical Research Council MR/M020606/1; MR/M020606/2 Cancer Research UK C63579/A26206 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 22 Jan 2021 07:27 |
Last Modified: | 27 Jan 2022 07:16 |
Status: | Published |
Publisher: | Cell Press (Elsevier) |
Refereed: | Yes |
Identification Number: | 10.1016/j.bpj.2020.12.024 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:170360 |