Wang, Yue orcid.org/0000-0002-2482-005X, Reardon, Christopher P., Read, Nicholas et al. (5 more authors) (2020) Attachment and antibiotic response of early-stage biofilms studied using resonant hyperspectral imaging. npj Biofilms and Microbiomes. 57. ISSN 2055-5008
Abstract
Many bacterial species readily develop biofilms that act as a protective matrix against external challenge, e.g., from antimicrobial treatment. Therefore, biofilms are often responsible for persistent and recurring infections. Established methods for studying biofilms are either destructive or focus on the biofilm’s surface. A non-destructive method that is sensitive to the underside of the biofilm is highly desirable, as it allows studying the penetration of antibiotics through the film. Here, we demonstrate that the high surface sensitivity of resonant hyperspectral imaging provides this capability. The method allows us to monitor the early stages of Escherichia coli biofilm formation, cell attachment and microcolony formation, in-situ and in real-time. We study the response of the biofilm to a number of different antibiotics and verify our observations using confocal microscopy. Based on this ability to closely monitor the surface-bound cells, resonant hyperspectral imaging gives new insights into the antimicrobial resistance of biofilms.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2020 |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > York Institute for Materials Research The University of York > Faculty of Sciences (York) > Physics (York) The University of York > Faculty of Sciences (York) > Centre for Immunology and Infection (CII) (York) The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Hull York Medical School (York) |
Depositing User: | Pure (York) |
Date Deposited: | 18 Dec 2020 15:00 |
Last Modified: | 01 Dec 2024 01:17 |
Published Version: | https://doi.org/10.1038/s41522-020-00169-1 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1038/s41522-020-00169-1 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:169269 |
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Description: Attachment and antibiotic response of early-stage biofilms studied using resonant hyperspectral imaging
Licence: CC-BY 2.5