Walker, H., Burrell, M., Flatley, J. et al. (1 more author) (2017) A metabolite profiling method for diagnosis of precancerous cervical lesions and HPV persistence. Bioanalysis, 9 (8). pp. 601-608. ISSN 1757-6180
Abstract
Aim: With the advent of rapid metabolic profiling techniques and of portable mass spectrometers we examined whether cells distinguished by their cytology and persistence of human papillomavirus infection, could be easily differentiated by their metabolite profile. Materials & methods: Direct injection electrospray mass spectrometry was used in a nontargeted double-blind experiment. Samples were collected from women diagnosed with one of two grades of cervical cytology and exhibiting either human papilloma virus persistence or clearance. Cell extracts were prepared using a DNA extraction procedure and the resulting supernatant, normally discarded, was analyzed. Data were interpreted using principal component analysis. Results: The results indicate strongly that a simple metabolite profiling method could be used to rapidly identify women at increased risk of cervical cancer.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Future Science Ltd. This is an author-produced version of a paper subsequently published in Bioanalysis. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | cervical cancer; HPV; mass spectrometry; metabolite profiling; metabolomics |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Sheffield Teaching Hospitals |
Funding Information: | Funder Grant number WORLD CANCER RESEARCH FUND INTERNATIONAL 2006/18 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 16 Dec 2020 13:47 |
Last Modified: | 17 Dec 2020 16:20 |
Status: | Published |
Publisher: | Future Science Group |
Refereed: | Yes |
Identification Number: | 10.4155/bio-2017-0012 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:169097 |