Bao, P orcid.org/0000-0002-7875-7420, Paterson, DA orcid.org/0000-0001-9425-720X, Peyman, SA orcid.org/0000-0002-1600-5100 et al. (5 more authors) (2021) Production of Giant Unilamellar Vesicles and Encapsulation of Nematic Lyotropic Liquid Crystals. Soft Matter, 17. pp. 2234-2241. ISSN 1744-683X
Abstract
We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs)viawater/octanol-lipid/water double emulsion droplets. At a high enough lipid concentration we show that thede-wetting of the octanol from these droplets occurs spontaneously (off-chip) without the need to useshear to aid the de-wetting process. The resultant mixture of octanol droplets and GUVs can beseparated by making use of the buoyancy of the octanol. A simpler microfluidic device and pumpsystem can be employed and, because of the higher flow-rates and much higher rate of formation ofthe double emulsion droplets (B1500 s 1compared to up toB75 s 1), it is easier to make largernumbers of GUVs and larger volumes of solution. Because of the potential for using GUVs thatincorporate lyotropic nematic liquid crystals in biosensors we have used this method to make GUVs thatincorporate the nematic phases of sunset yellow and disodium chromoglycate. However, the phasebehaviour of these lyotropic liquid crystals is quite sensitive to concentration and we found that there isan unexpected spread in the concentration of the contents of the GUVs obtained.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © The Royal Society of Chemistry 2021. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Soft Matter Physics (Leeds) |
Funding Information: | Funder Grant number EPSRC (Engineering and Physical Sciences Research Council) EP/P024041/1 EPSRC (Engineering and Physical Sciences Research Council) EP/P023266/1 NIHR National Inst Health Research Not Known Leeds Teaching Hospitals NHS Trust NIHR Surgical MIC MF EPSRC (Engineering and Physical Sciences Research Council) EP/S029214/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 11 Dec 2020 12:48 |
Last Modified: | 25 Jun 2023 22:31 |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Identification Number: | 10.1039/d0sm01684e |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:168924 |