Weissman, D., Alameh, M.-G., de Silva, T. orcid.org/0000-0002-6498-9212 et al. (22 more authors) (2021) D614G Spike mutation increases SARS CoV-2 susceptibility to neutralization. Cell Host & Microbe, 29 (1). 23-31.e4. ISSN 1931-3128
Abstract
The SARS-CoV-2 Spike protein acquired a D614G mutation early in the pandemic that confers greater infectivity and is now the globally dominant form. To determine whether D614G might also mediate neutralization-escape that could compromise vaccine efficacy, sera from Spike-immunized mice, nonhuman primates and humans were evaluated for neutralization of pseudoviruses bearing either D614 or G614 spike. In all cases, the G614 pseudovirus was moderately more susceptible to neutralization. The G614 pseudovirus also was more susceptible to neutralization by receptor binding domain (RBD) monoclonal antibodies and convalescent sera from people infected with either form of the virus. Negative stain electron microscopy revealed a higher percentage of the 1-RBD “up” conformation in the G614 spike, suggesting increased epitope exposure as a mechanism of enhanced vulnerability to neutralization. Based on these findings, the D614G mutation is not expected to be an obstacle for current vaccine development.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 Published by Elsevier Inc. This is an author produced version of a paper subsequently published in Cell Host & Microbe. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Sheffield Teaching Hospitals |
Funding Information: | Funder Grant number COVID-19 Genomics UK Consortium N/A |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 09 Dec 2020 14:12 |
Last Modified: | 01 Feb 2022 14:44 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.chom.2020.11.012 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:168889 |
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Licence: CC-BY-NC-ND 4.0