Ashwin, Helen, Sadlova, Jovana, Vojtkova, Barbora et al. (14 more authors) (2021) Characterization of a new Leishmania major strain for use in a controlled human infection model. Nature Communications. 215. p. 215. ISSN 2041-1723
Abstract
Leishmaniasis is widely regarded as a vaccine-preventable disease, but the costs required to reach pivotal Phase 3 studies and uncertainty about which candidate vaccines should be progressed into human studies significantly limits progress in vaccine development for this neglected tropical disease. Controlled human infection models (CHIMs) provide a pathway for accelerating vaccine development and to more fully understand disease pathogenesis and correlates of protection. Here, we describe the isolation, characterization and GMP manufacture of a new clinical strain of Leishmania major. Two fresh strains of L. major from Israel were initially compared by genome sequencing, in vivo infectivity and drug sensitivity in mice, and development and transmission competence in sand flies, allowing one to be selected for GMP production. This study addresses a major roadblock in the development of vaccines for leishmaniasis, providing a key resource for CHIM studies of sand fly transmitted cutaneous leishmaniasis.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2021 |
Keywords: | Animals,Disease Models, Animal,Humans,Insect Vectors/parasitology,Israel,Leishmania major/genetics,Leishmaniasis, Cutaneous/parasitology,Mice, Inbred BALB C,Parasites/genetics,Phylogeny,Psychodidae/parasitology,Whole Genome Sequencing,Mice |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Hull York Medical School (York) |
Depositing User: | Pure (York) |
Date Deposited: | 07 Dec 2020 17:50 |
Last Modified: | 01 Dec 2024 01:17 |
Published Version: | https://doi.org/10.1038/s41467-020-20569-3 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1038/s41467-020-20569-3 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:168769 |
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Description: Characterization of a new Leishmania major strain for use in a controlled human infection model
Licence: CC-BY 2.5