Kirkby, CJ orcid.org/0000-0002-5262-1568, Gala De Pablo, J orcid.org/0000-0003-0557-9632, Tinkler-Hundal, E et al. (3 more authors) (2020) Developing a Raman spectroscopy-based tool to stratify patient response to pre-operative radiotherapy in rectal cancer. The Analyst. ISSN 0003-2654
Abstract
Rectal cancer patients frequently receive pre-operative radiotherapy (RT), prior to surgical resection. However, colorectal cancer is heterogeneous and the degree of tumour response to pre-operative RT is highly variable. There are currently no clinically approved methods of predicting response to RT, and a significant proportion of patients will show no clinical benefit, despite enduring the side-effects. We evaluated the use of Raman spectroscopy (RS), a non-destructive technique able to provide the unique chemical fingerprint of tissues, as a potential tool to stratify patient response to pre-operative RT. Raman measurements were obtained from the formalin-fixed, paraffin-embedded (FFPE) pre-treatment biopsy specimens of 20 rectal cancer patients who received pre-operative RT. A principal component analysis and linear discriminant analysis algorithm was able to classify patient response to pre-operative RT as good or poor, with an accuracy of 86.04 ± 0.14% (standard error). Patients with a good response to RT showed greater contributions from protein-associated peaks, whereas patients who responded poorly showed greater lipid contributions. These results demonstrate that RS is able to reliably classify tumour response to pre-operative RT from FFPE biopsies and highlights its potential to guide personalised cancer patient treatment.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Royal Society of Chemistry 2020. This is an open access article under the terms of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) |
Funding Information: | Funder Grant number Yorkshire Cancer Research L394 MRC (Medical Research Council) MR/M009084/1 EPSRC (Engineering and Physical Sciences Research Council) EP/P023266/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 Nov 2020 17:01 |
Last Modified: | 08 Jan 2025 12:45 |
Status: | Published online |
Publisher: | Royal Society of Chemistry (RSC) |
Identification Number: | 10.1039/d0an01803a |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:167941 |
Commentary/Response Threads
- Kirkby, CJ, Gala De Pablo, J, Tinkler-Hundal, E, Wood, HM, Evans, SD and West, NP Developing a Raman spectroscopy-based tool to stratify patient response to pre-operative radiotherapy in rectal cancer. (deposited 13 Nov 2020 17:01) [Currently Displayed]