Bagdonas, Haroldas, Ungar, Daniel orcid.org/0000-0002-9852-6160 and Agirre, Jon orcid.org/0000-0002-1086-0253 (2020) Leveraging glycomics data in glycoprotein 3D structure validation with Privateer. Beilstein Journal of Organic Chemistry. pp. 2523-2533. ISSN 1860-5397
Abstract
The heterogeneity, mobility and complexity of glycans in glycoproteins have been, and currently remain, significant challenges in structural biology. These aspects present unique problems to the two most prolific techniques: X-ray crystallography and cryo-electron microscopy. At the same time, advances in mass spectrometry have made it possible to get deeper insights on precisely the information that is most difficult to recover by structure solution methods: the full-length glycan composition, including linkage details for the glycosidic bonds. The developments have given rise to glycomics. Thankfully, several large scale glycomics initiatives have stored results in publicly available databases, some of which can be accessed through API interfaces. In the present work, we will describe how the Privateer carbohydrate structure validation software has been extended to harness results from glycomics projects, and its use to greatly improve the validation of 3D glycoprotein structures.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Copyright © 2020, Bagdonas et al.; licensee Beilstein-Institut. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Funding Information: | Funder Grant number THE ROYAL SOCIETY RGF\R1\181006 |
Depositing User: | Pure (York) |
Date Deposited: | 12 Nov 2020 11:20 |
Last Modified: | 16 Oct 2024 17:05 |
Published Version: | https://doi.org/10.3762/bjoc.16.204 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.3762/bjoc.16.204 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:167939 |