van Roermund, CWT, IJlst, L, Baker, A orcid.org/0000-0003-2181-4057 et al. (3 more authors) (2021) The Saccharomyces cerevisiae ABC subfamily D transporter Pxa1/Pxa2p co‐imports CoASH into the peroxisome. FEBS Letters, 595 (6). 1873-3468.13974. pp. 763-772. ISSN 0014-5793
Abstract
ATP-binding cassette (ABC) subfamily D transporters are important for the uptake of fatty acids and other beta-oxidation substrates into peroxisomes. Genetic and biochemical evidence indicates that the transporters accept fatty acyl-coenzyme A that is cleaved during the transport cycle and then re-esterified in the peroxisomal lumen. However, it is not known whether free coenzyme A (CoA) is released inside or outside the peroxisome. Here we have used Saccharomyces cerevisiae and isolated peroxisomes to demonstrate that free CoA is released in the peroxisomal lumen. Thus, ABC subfamily D transporter provide an import pathway for free CoA that controls peroxisomal CoA homeostasis and tunes metabolism according to the cell’s demands.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 Federation of European Biochemical Societies. This is the peer reviewed version of the following article: van Roermund, C.W.T., IJlst, L., Baker, A., Wanders, R.J.A., Theodoulou, F.L. and Waterham, H.R. (2021), The Saccharomyces cerevisiae ABC subfamily D transporter Pxa1/Pxa2p co-imports CoASH into the peroxisome. FEBS Lett, 595: 763-772., which has been published in final form at https://doi.org/10.1002/1873-3468.13974. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
Keywords: | ABC transporter; acyl-CoA; coenzyme A; coenzymehomeostasis; peroxisome; thioesterase |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Plant Cell & Cellular Biology (Leeds) |
Funding Information: | Funder Grant number BBSRC (Biotechnology & Biological Sciences Research Council) BB/L001012/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 03 Nov 2020 13:16 |
Last Modified: | 31 May 2022 14:18 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/1873-3468.13974 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:167460 |