Chong, Zheng-Shan, Wright, Gavin J orcid.org/0000-0003-0537-0863 and Sharma, Sumana (2020) Investigating cellular recognition using crispr/cas9 genetic screening. TRENDS IN CELL BIOLOGY. pp. 619-627. ISSN 1879-3088
Abstract
Neighbouring cells can recognise and communicate with each other by direct binding between cell surface receptor and ligand pairs. Examples of cellular recognition events include pathogen entry into a host cell, sperm-egg fusion, and self/nonself discrimination by the immune system. Despite growing appreciation of cell surface recognition molecules as potential therapeutic targets, identifying key factors contributing to cellular recognition remains technically challenging to perform on a genome-wide scale. Recently, genome-scale clustered regularly interspaced short palindromic repeats (CRISPR) knockout or activation (CRISPR-KO/CRISPRa) screens have been applied to identify the molecular determinants of cellular recognition. In this review, we discuss how CRISPR-KO/CRISPRa screening has contributed to our understanding of cellular recognition processes, and how it can be applied to investigate these important interactions in a range of biological contexts. Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 The Authors. |
Keywords: | cellular genetics,staffpaper |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Hull York Medical School (York) |
Depositing User: | Pure (York) |
Date Deposited: | 20 Oct 2020 11:50 |
Last Modified: | 16 Oct 2024 17:01 |
Published Version: | https://doi.org/10.1016/j.tcb.2020.05.005 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.tcb.2020.05.005 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:166936 |