Lamas-Toranzo, Ismael, Hamze, Julieta G, Bianchi, Enrica et al. (9 more authors) (2020) TMEM95 is a sperm membrane protein essential for mammalian fertilization. eLife. e53913. ISSN 2050-084X
Abstract
The fusion of gamete membranes during fertilization is an essential process for sexual reproduction. Despite its importance, only three proteins are known to be indispensable for sperm-egg membrane fusion: the sperm proteins IZUMO1 and SPACA6, and the egg protein JUNO. Here we demonstrate that another sperm protein, TMEM95, is necessary for sperm-egg interaction. TMEM95 ablation in mice caused complete male-specific infertility. Sperm lacking this protein were morphologically normal exhibited normal motility, and could penetrate the zona pellucida and bind to the oolemma. However, once bound to the oolemma, TMEM95-deficient sperm were unable to fuse with the egg membrane or penetrate into the ooplasm, and fertilization could only be achieved by mechanical injection of one sperm into the ooplasm, thereby bypassing membrane fusion. These data demonstrate that TMEM95 is essential for mammalian fertilization. © 2020, Lamas-Toranzo et al.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Keywords: | staffpaper |
Dates: |
|
Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Hull York Medical School (York) |
Depositing User: | Pure (York) |
Date Deposited: | 20 Oct 2020 09:10 |
Last Modified: | 01 Dec 2024 01:16 |
Published Version: | https://doi.org/10.7554/eLife.53913 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.7554/eLife.53913 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:166917 |