Chong, Zheng-Shan, Ohnishi, Shuhei, Yusa, Kosuke et al. (1 more author) (2018) Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Genome biology. 205. ISSN 1474-760X
Abstract
Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be unambiguously identified when used in pools or as individual binding probes. We apply this technique to identify ligands for the adhesion G-protein-coupled receptors and show that the Nogo myelin-associated inhibitory proteins are ligands for ADGRB1. This method will enable extracellular receptor-ligand identification on a genome-wide scale.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Hull York Medical School (York) |
Depositing User: | Pure (York) |
Date Deposited: | 19 Oct 2020 16:10 |
Last Modified: | 16 Oct 2024 17:01 |
Published Version: | https://doi.org/10.1186/s13059-018-1581-3 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1186/s13059-018-1581-3 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:166890 |