Harvey, D, Bray, G, Zamberlan, F et al. (3 more authors) (2020) Cyclo(RGDfK) Functionalized Spider Silk Cell Scaffolds: Significantly Improved Performance in Just One Click. Macromolecular Bioscience, 20 (12). 200255. ISSN 1616-5187
Abstract
Recombinant spider silk has the potential to provide a new generation of biomaterial scaffolds as a result of its degree of biocompatibility and lack of immunogenicity. These recombinant biomaterials are, however, reported to exhibit poor cellular adhesion which limits their potential for use in applications such as tissue engineering and regenerative medicine. In this study, a simple chemical functionalization approach is described that specifically addresses this issue and significantly improves the adhesion of human mesenchymal stem cells (CiMSCs) to a recombinant spider silk biomaterial. This utilizes copper‐catalyzed or strain‐promoted azide–alkyne cycloaddition (CuAAC/SPAAC) “click” chemistry to covalently attach cyclo(RGDfK) peptides to the azide group of l‐azidohomoalanine, a methionine analogue previously site specifically incorporated into the primary sequence of a thioredoxin (TRX)‐tagged silk fusion protein, TRX‐4RepCT, to give TRX3Aha‐4RepCT3Aha. This method is used to produce cyclo(RGDfK) functionalized films and macroscopic fibers. Over 24 h, cyclo(RGDfK) functionalized TRX3Aha‐4RepCT3Aha films and 4RepCT3Aha fibers display significantly improved performance in CiMSC culture, yielding far greater cell numbers than the controls. This approach circumvents the previously observed lack of cell adhesion, thus allowing spider silk derived biomaterials to be used where such adhesion is critical, in tissue engineering, regenerative medicine and wound healing.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2020 The Authors. Published by Wiley‐VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) |
Keywords: | Aha, bioorthogonal, click chemistry, CuAAC, RGD, silk, strain-promoted azide-alkyne cycloaddition |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 Oct 2020 13:45 |
Last Modified: | 25 Jun 2023 22:27 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/mabi.202000255 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:166626 |