Adamson, H and Jeuken, LJC orcid.org/0000-0001-7810-3964 (2020) Engineering Protein Switches for Rapid Diagnostic Tests. ACS Sensors, 5 (10). pp. 3001-3012. ISSN 2379-3694
Abstract
Biological signaling pathways are underpinned by protein switches that sense and respond to molecular inputs. Inspired by nature, engineered protein switches have been designed to directly transduce analyte binding into a quantitative signal in a simple, wash-free, homogeneous assay format. As such, they offer great potential to underpin point-of-need diagnostics that are needed across broad sectors to improve access, costs, and speed compared to laboratory assays. Despite this, protein switch assays are not yet in routine diagnostic use, and a number of barriers to uptake must be overcome to realize this potential. Here, we review the opportunities and challenges in engineering protein switches for rapid diagnostic tests. We evaluate how their design, comprising a recognition element, reporter, and switching mechanism, relates to performance and identify areas for improvement to guide further optimization. Recent modular switches that enable new analytes to be targeted without redesign are crucial to ensure robust and efficient development processes. The importance of translational steps toward practical implementation, including integration into a user-friendly device and thorough assay validation, is also discussed.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2020 American Chemical Society. This is an open access article under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) (https://creativecommons.org/licenses/by/4.0/) |
Keywords: | protein engineering; protein switch; wash-free; homogeneous assay; immunoassay; rapid diagnostics; point-of-care; biosensors |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) |
Funding Information: | Funder Grant number MRC (Medical Research Council) MR/N029976/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 02 Oct 2020 12:47 |
Last Modified: | 25 Jun 2023 22:26 |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/acssensors.0c01831 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:166128 |