Farrington, J., Seward, E.P. orcid.org/0000-0001-5311-1343 and Peachell, P.T. (2020) Mast cell purification protocols. In: Gibbs, B.F. and Falcone, F.H., (eds.) Basophils and Mast Cells : Methods and Protocols. Methods in Molecular Biology (2163). Springer , pp. 49-56. ISBN 9781071606957
Abstract
Studying a tissue-specific mast cell can be of particular benefit given the heterogeneity that is known to exist among mast cells isolated or developed from different sources. Methods for isolating mast cells from a variety of tissues have been in existence for a number of years although, over time, these methodologies have been refined. We have had considerable experience studying mast cells isolated from human lung tissue. It is for this reason that, in this chapter, we provide detailed methods for the isolation and purification of human lung mast cells. However, it should be noted that the methods that are described in this chapter are generally applicable to the isolation of mast cells from different tissues, and this will also be discussed.
Metadata
Item Type: | Book Section |
---|---|
Authors/Creators: |
|
Editors: |
|
Copyright, Publisher and Additional Information: | © 2020 Springer Science+Business Media, LLC. |
Keywords: | CD117; Dynabeads®; Mast cells |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 30 Sep 2020 09:09 |
Last Modified: | 30 Sep 2020 09:09 |
Status: | Published |
Publisher: | Springer |
Series Name: | Methods in Molecular Biology |
Refereed: | Yes |
Identification Number: | 10.1007/978-1-0716-0696-4_3 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:165981 |