De Boer, Casper, McGregor, Nicholas George Sarre, Peterse, Evert et al. (10 more authors) (2020) Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases. RSC Chemical Biology. pp. 148-155. ISSN 2633-0679
Abstract
Cellulases and related β-1,4-glucanases are essential components of lignocellulose-degrading enzyme mixtures. The detection of β-1,4-glucanase activity typically relies on monitoring the breakdown of purified lignocellulose-derived substrates or synthetic chromogenic substrates, limiting the activities which can be detected and complicating the tracing of activity back to specific components within complex enzyme mixtures. As a tool for the rapid detection and identification of β-1,4-glucanases, a series of glycosylated cyclophellitol inhibitors mimicking β-1,4-glucan oligosaccharides have been synthesised. These compounds are highly efficient inhibitors of HiCel7B, a well-known GH7 endo -β-1,4-glucanase. An elaborated activity-based probe facilitated the direct detection and identification of β-1,4-glucanases within a complex fungal secretome without any detectable cross-reactivity with β- d -glucosidases. These probes and inhibitors add valuable new capacity to the growing toolbox of cyclophellitol-derived probes for the activity-based profiling of biomass-degrading enzymes.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Royal Society of Chemistry 2020 |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Funding Information: | Funder Grant number THE ROYAL SOCIETY RSRP\R\210004 BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/R001162/1 |
Depositing User: | Pure (York) |
Date Deposited: | 02 Sep 2020 09:00 |
Last Modified: | 16 Oct 2024 16:54 |
Published Version: | https://doi.org/10.1039/D0CB00045K |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1039/D0CB00045K |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:164982 |
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Description: Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases
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