Sanderson, Thomas, Black, Conor, Southwell, James et al. (7 more authors) (2020) A Salmochelin S4-inspired Ciprofloxacin Trojan Horse Conjugate. ACS Infectious Diseases. 2532–2541. ISSN 2373-8227
Abstract
A novel ciprofloxacin-siderophore Trojan Horse antimicrobial was prepared by incorporating key design features of salmochelin S4, a stealth siderophore that evades mammalian siderocalin capture via its glycosylated catechol units. As-sessment of the antimicrobial activity of the conjugate revealed that attachment of the salmochelin mimic resulted in decreased potency, compared to ciprofloxacin, against two Escherichia coli strains, K12 and Nissle 1917, in both iron-replete and deplete conditions. This observation could be attributed to a combination of reduced DNA gyrase inhibi-tion, as confirmed by in vitro DNA gyrase assays, and reduced bacterial uptake. Uptake was monitored using radio-labelling with iron-mimetic 67Ga3+, which revealed limited cellular uptake in E. coli K12. In contrast, previously reported staphyloferrin-based conjugates displayed measurable uptake in analogous 67Ga3+, labelling studies. These results suggest that in designing Trojan Horse antimicrobials, the choice of siderophore and the nature and length of the link-er remains a significant challenge.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 American Chemical Society. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Funding Information: | Funder Grant number EPSRC EP/L024829/1 UNSPECIFIED EP/T007338/1 |
Depositing User: | Pure (York) |
Date Deposited: | 25 Aug 2020 16:40 |
Last Modified: | 16 Oct 2024 16:52 |
Published Version: | https://doi.org/10.1021/acsinfecdis.0c00568 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1021/acsinfecdis.0c00568 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:164830 |