Nicholson, D orcid.org/0000-0001-5404-8251, Edwards, TA orcid.org/0000-0002-1467-3674, O'Neill, AJ orcid.org/0000-0003-3222-5493 et al. (1 more author) (2020) Structure of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with clinically-relevant antibiotics. Structure, 28 (10). pp. 1087-1100. ISSN 0969-2126
Abstract
Acinetobacter baumannii is a Gram-negative bacterium primarily associated with hospital-acquired, often multidrug-resistant (MDR) infections. The ribosome-targeting antibiotics amikacin and tigecycline are among the limited arsenal of drugs available for treatment of such infections. We present high-resolution structures of the 70S ribosome from A. baumannii in complex with these antibiotics, as determined by cryoelectron microscopy. Comparison with the ribosomes of other bacteria reveals several unique structural features at functionally important sites, including around the exit of the polypeptide tunnel and the periphery of the subunit interface. The structures also reveal the mode and site of interaction of these drugs with the ribosome. This work paves the way for the design of new inhibitors of translation to address infections caused by MDR A. baumannii.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 The Authors. This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Ribosome; antibiotics; cryoelectron microscopy; antimicrobial resistance; structural biology |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Cryo EM, Image Processing (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Crystallography (Leeds) |
Funding Information: | Funder Grant number Wellcome Trust 203743/Z/16/Z |
Depositing User: | Symplectic Publications |
Date Deposited: | 26 Aug 2020 13:41 |
Last Modified: | 25 Feb 2025 14:25 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.str.2020.08.004 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:164782 |