Jalal, Adam, Tran, Ngat, Stevenson, Clare et al. (6 more authors) (2020) Diversification of DNA-Binding Specificity by Permissive and Specificity-Switching Mutations in the ParB/Noc Protein Family. Cell reports. 107928. ISSN 2211-1247
Abstract
Specific interactions between proteins and DNA are essential to many biological processes. Yet, it remains unclear how the diversification in DNA-binding specificity was brought about, and the mutational paths that led to changes in specificity are unknown. Using a pair of evolutionarily related DNA-binding proteins, each with a different DNA preference (ParB [Partitioning Protein B] and Noc [Nucleoid Occlusion Factor], which both play roles in bacterial chromosome maintenance), we show that specificity is encoded by a set of four residues at the protein-DNA interface. Combining X-ray crystallography and deep mutational scanning of the interface, we suggest that permissive mutations must be introduced before specificity-switching mutations to reprogram specificity and that mutational paths to new specificity do not necessarily involve dual-specificity intermediates. Overall, our results provide insight into the possible evolutionary history of ParB and Noc and, in a broader context, might be useful for understanding the evolution of other classes of DNA-binding proteins.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 The Author(s) |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Physics (York) |
Funding Information: | Funder Grant number EPSRC EP/N027639/1 |
Depositing User: | Pure (York) |
Date Deposited: | 06 Aug 2020 09:10 |
Last Modified: | 22 Jan 2025 00:14 |
Published Version: | https://doi.org/10.1016/j.celrep.2020.107928 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.celrep.2020.107928 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:164157 |