Moody, N.R., Christin, P.-A. and Reid, J.D. orcid.org/0000-0003-4921-1047 (2020) Kinetic modifications of C4 PEPC are qualitatively convergent, but larger in Panicum than in Flaveria. Frontiers in Plant Science, 11. 1014. ISSN 1664-462X
Abstract
C4 photosynthesis results from a set of anatomical features and biochemical components that act together to concentrate CO2 within the leaf and boost productivity. This complex trait evolved independently many times, resulting in various realizations of the phenotype, but in all C4 plants the primary fixation of atmospheric carbon is catalyzed by phosphoenolpyruvate carboxylase. Comparisons of C4 and non-C4 PEPC from a few closely related species suggested that the enzyme was modified to meet the demands of the C4 cycle. However, very few C4 groups have been investigated, hampering general conclusions. To test the hypothesis that distant C4 lineages underwent convergent biochemical changes, we compare the kinetic variation between C4 and non-C4 PEPC from a previously assessed young lineage (Flaveria, Asteraceae) with those from an older lineage found within the distantly related grass family (Panicum). Despite the evolutionary distance, the kinetic changes between the non-C4 and C4 PEPC are qualitatively similar, with a decrease in sensitivity for inhibitors, an increased specificity (kcat/Km) for bicarbonate, and a decreased specificity (kcat/Km) for PEP. The differences are more pronounced in the older lineage Panicum, which might indicate that optimization of PEPC for the C4 context increases with evolutionary time.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 Moody, Christin and Reid. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) http://creativecommons.org/licenses/by/4.0/. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: | C4 photosynthesis; carbon fixation; enzyme evolution; feedback inhibition; kinetics; phosphoenolpyruvate carboxylase |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Funding Information: | Funder Grant number BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL BB/M000265/1 EUROPEAN COMMISSION - HORIZON 2020 638333 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 17 Jul 2020 13:56 |
Last Modified: | 17 Jul 2020 13:56 |
Status: | Published |
Publisher: | Frontiers Media SA |
Refereed: | Yes |
Identification Number: | 10.3389/fpls.2020.01014 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:163389 |