Greig, J. and Bulgakova, N. orcid.org/0000-0002-3780-8164 (2020) Interplay between actomyosin and E-cadherin dynamics regulates cell shape in the Drosophila embryonic epidermis. Journal of Cell Science, 133 (15). jcs242321. ISSN 0021-9533
Abstract
Precise regulation of cell shape is vital for building functional tissues. Here, we study the mechanisms which lead to the formation of highly elongated anisotropic epithelial cells in the Drosophila epidermis. We demonstrate that this cell shape is the result of two counteracting mechanisms at the cell surface which regulate the degree of elongation: actomyosin, which inhibits cell elongation downstream of RhoA signalling, and intercellular adhesion, modulated via clathrin-mediated endocytosis of E-cadherin, which promotes cell elongation downstream of the GTPase Arf1. We show that these two mechanisms do not act independently but are interconnected, with RhoA signalling reducing Arf1 recruitment to the plasma membrane. Additionally, cell adhesion itself regulates both mechanisms: p120-catenin, a regulator of intercellular adhesion, promotes the activity of both Arf1 and RhoA. Altogether, we uncover a complex network of interactions between cell-cell adhesion, the endocytic machinery, and the actomyosin cortex, and demonstrate how this network regulates cell shape in an epithelial tissue in vivo.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 The Author(s). Published by The Company of Biologists Ltd. This is an author-produced version of a paper subsequently published in Journal of Cell Science. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | cell shape; epithelium; adhesion; actin; p120ctn |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Funding Information: | Funder Grant number BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL BB/P007503/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 08 Jul 2020 10:12 |
Last Modified: | 20 Jan 2022 08:56 |
Status: | Published |
Publisher: | Company of Biologists |
Refereed: | Yes |
Identification Number: | 10.1242/jcs.242321 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:163001 |