Greig, J. and Bulgakova, N. orcid.org/0000-0002-3780-8164 (2020) Arf6 determines tissue architecture by stabilizing intercellular adhesion. Philosophical Transactions B: Biological Sciences, 375 (1809). 20190682. ISSN 0962-8436
Abstract
Correct cell shape is indispensable for tissue architecture, with cell shape being determined by cortical actin and surface adhesion. The role of adhesion in remodeling tissue is to counteract the deformation of cells by force, resulting from actomyosin contractility, and to maintain tissue integrity. The dynamics of this adhesion is critical to the processes of cell shape formation and maintenance. Here, we show that the trafficking molecule, Arf6, has a direct impact on cell elongation, by acting to stabilize E-cadherin-based adhesion complexes at the cell surface, in addition to its canonical role in endocytosis. We demonstrate that these functions of Arf6 are dependent on the molecule Flotillin1, which recruits Arf6 to the plasma membrane. Our data suggest that Arf6 and Flotillin1 operate in a pathway distinct from clathrin-mediated endocytosis. Altogether, we demonstrate that Arf6- and Flotillin1-dependent regulation of the dynamics of cell adhesion contributes to molding tissue in vivo.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 The Author(s). This is an author-produced version of a paper subsequently published in Philosophical Transactions B: Biological Sciences. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Arf6; cell shape; epithelium; adhesion; actin; endocytosis |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Funding Information: | Funder Grant number BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL BB/P007503/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 08 Jul 2020 10:02 |
Last Modified: | 03 Sep 2020 09:22 |
Status: | Published |
Publisher: | Royal Society, The |
Refereed: | Yes |
Identification Number: | 10.1098/rstb.2019.0682 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:162999 |