Gao, Hong, Taylor, Gabrielle, Evans, Stephanie K. et al. (2 more authors) (2020) Application of serine integrases for secondary metabolite pathway assembly in Streptomyces. Synthetic and Systems Biotechnology. pp. 111-119. ISSN 2405-805X
Abstract
Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can be applied for use in vitro for the heterologous expression of complex biosynthesis pathways in Streptomyces spp., the major producers of useful bioactive natural products. The results show that multiple orthogonal serine integrases efficiently assemble the genes from a complex biosynthesis pathway in a single in vitro recombination reaction, potentially permitting a versatile combinatorial assembly approach. Furthermore, the assembly strategy also permitted the incorporation of a well-characterised promoter upstream of each gene for expression in a heterologous host. The results demonstrate how site-specific recombination based on orthogonal serine integrases can be applied in Streptomyces spp.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 The Author(s). |
Keywords: | Erythromycin,Pathway assembly,Serine integrase,Streptomyces |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York |
Depositing User: | Pure (York) |
Date Deposited: | 30 Jun 2020 13:50 |
Last Modified: | 19 Nov 2024 00:38 |
Published Version: | https://doi.org/10.1016/j.synbio.2020.05.006 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.synbio.2020.05.006 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:162639 |
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