Lerner, E., Ambrose, B., Barth, A. et al. (23 more authors) (Submitted: 2020) The FRET-based structural dynamics challenge -- community contributions to consistent and open science practices. arXiv. (Submitted)
Abstract
Single-molecule Förster resonance energy transfer (smFRET) has become a mainstream technique for probing biomolecular structural dynamics. The rapid and wide adoption of the technique by an ever-increasing number of groups has generated many improvements and variations in the technique itself, in methods for sample preparation and characterization, in analysis of the data from such experiments, and in analysis codes and algorithms. Recently, several labs that employ smFRET have joined forces to try to bring the smFRET community together in adopting a consensus on how to perform experiments and analyze results for achieving quantitative structural information. These recent efforts include multi-lab blind-tests to assess the accuracy and precision of smFRET between different labs using different procedures, the formal assembly of the FRET community and development of smFRET procedures to be considered for entries in the wwPDB. Here we delve into the different approaches and viewpoints in the field. This position paper describes the current "state-of-the field", points to unresolved methodological issues for quantitative structural studies, provides a set of 'soft recommendations' about which an emerging consensus exists, and a list of resources that are openly available. To make further progress, we strongly encourage 'open science' practices. We hope that this position paper will provide a roadmap for newcomers to the field, as well as a reference for seasoned practitioners.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 The Author(s). Article available under the terms of the Creative Commons Attribution-NonCommercial ShareAlike Licence (http://creativecommons.org/licenses/by-nc-sa/4.0/). |
Keywords: | q-bio.BM; q-bio.BM; physics.bio-ph |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Funding Information: | Funder Grant number The Royal Society RGS\R2\180405 Biotechnology and Biological Sciences Research Council BB/T008032/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 23 Jun 2020 06:57 |
Last Modified: | 23 Jun 2020 21:32 |
Published Version: | https://arxiv.org/abs/2006.03091 |
Status: | Submitted |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:162218 |