Hoh, Soon Wen orcid.org/0000-0003-1039-8000, Burnley, Tom and Cowtan, Kevin orcid.org/0000-0002-0189-1437 (2020) Current approaches for automated model building into cryo-EM maps using Buccaneer with CCP-EM. Acta crystallographica. Section D, Structural biology. pp. 531-541. ISSN 2059-7983
Abstract
This work focuses on the use of the existing protein-model-building software Buccaneer to provide structural interpretation of electron cryo-microscopy (cryo-EM) maps. Originally developed for application to X-ray crystallography, the necessary steps to optimise the usage of Buccaneer with cryo-EM maps are shown. This approach has been applied to the data sets of 208 cryo-EM maps with resolutions of better than 4 Å. The results obtained also show an evident improvement in the sequencing step when the initial reference map and model used for crystallographic cases are replaced by a cryo-EM reference. All other necessary changes to settings in Buccaneer are implemented in the model-building pipeline from within the CCP-EM interface (as of version 1.4.0).
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Buccaneer,CCP-EM,Collaborative Computational Project for Electron cryo-Microscopy,Cryo-EM,Model building |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Funding Information: | Funder Grant number BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/P000517/1 |
Depositing User: | Pure (York) |
Date Deposited: | 12 Jun 2020 09:30 |
Last Modified: | 16 Oct 2024 16:40 |
Published Version: | https://doi.org/10.1107/S2059798320005513 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1107/S2059798320005513 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:161859 |