Chau, C, Actis, P orcid.org/0000-0002-7146-1854 and Hewitt, E orcid.org/0000-0002-6238-6303 (2020) Methods for protein delivery into cells: from current approaches to future perspectives. Biochemical Society Transactions, 48 (2). pp. 357-365. ISSN 0300-5127
Abstract
The manipulation of cultured mammalian cells by the delivery of exogenous macromolecules is one of the cornerstones of experimental cell biology. Although the transfection of cells with DNA expressions constructs that encode proteins is routine and simple to perform, the direct delivery of proteins into cells has many advantages. For example, proteins can be chemically modified, assembled into defined complexes and subject to biophysical analyses prior to their delivery into cells. Here, we review new approaches to the injection and electroporation of proteins into cultured cells. In particular, we focus on how recent developments in nanoscale injection probes and localized electroporation devices enable proteins to be delivered whilst minimizing cellular damage. Moreover, we discuss how nanopore sensing may ultimately enable the quantification of protein delivery at single-molecule resolution.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society This is an author produced version of an article published in Biochemical Society Transactions. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | electroporation, injection, nanopore sensing, nanotechnology, protein delivery |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 12 Jun 2020 11:02 |
Last Modified: | 08 Apr 2021 00:38 |
Status: | Published |
Publisher: | Portland Press |
Identification Number: | 10.1042/bst20190039 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:161824 |