Galloway, AF, Akhtar, J orcid.org/0000-0001-9193-0083, Marcus, SE orcid.org/0000-0002-5495-5684 et al. (3 more authors) (2020) Cereal root exudates contain highly structurally complex polysaccharides with soil‐binding properties. The Plant Journal, 103 (5). tpj.14852. pp. 1666-1678. ISSN 0960-7412
Abstract
Rhizosheaths function in plant‐soil interactions and are proposed to form due to a mix of soil particle entanglement in root hairs and the action of adhesive root exudates. The soil‐binding factors released into rhizospheres to form rhizosheaths have not been characterised. Analysis of the high molecular weight root exudates of both wheat and maize plants indicate the presence of complex, highly branched polysaccharide components with a wide range of galactosyl, glucosyl and mannosyl linkages that do not directly reflect cereal root cell wall polysaccharide structures. Periodate oxidation indicates that it is the carbohydrate components of the high molecular weight exudates that have soil‐binding properties. The root exudates contain xyloglucan (LM25), heteroxylan (LM11/LM27) and arabinogalactan‐protein epitopes (LM2) epitopes and sandwich‐ELISA evidence indicates that, in wheat particularly, these can be interlinked in multi‐polysaccharide complexes. Using wheat as a model, exudate‐binding monoclonal antibodies have enabled the tracking of polysaccharide release along root axes of young seedlings and their presence at root hair surfaces and in rhizosheaths. The observations indicate that specific root exudate polysaccharides, distinct from cell wall polysaccharides, are adhesive factors secreted by root axes and that they contribute to the formation and stabilization of cereal rhizosheaths.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020, Wiley. This is an author produced version of a paper published in The Plant Journal. This is the peer reviewed version of the following article: Galloway, AF, Akhtar, J , Marcus, SE et al. (3 more authors) (2020) Cereal root exudates contain highly structurally complex polysaccharides with soil‐binding properties. The Plant Journal, 103 (5). tpj.14852. pp. 1666-1678. ISSN 0960-7412, which has been published in final form at https://doi.org/10.1111/tpj.14852. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Plant‐soil interactions; polysaccharides; rhizosheaths; root exudates; Triticum aestivum; Zea mays |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 11 Jun 2020 12:01 |
Last Modified: | 19 Jul 2022 10:20 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/tpj.14852 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:161753 |