Smith, S, Zhu, S, Joos, L et al. (33 more authors) (2020) The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis. Molecular & Cellular Proteomics. ISSN 1535-9476
Abstract
Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-terminally encoded peptide 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Published under license by The American Society for Biochemistry and Molecular Biology, Inc. This is an author produced version of an article published in Molecular & Cellular Proteomics. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Arabidopsis; Developmental biology; Hormones; Label-free quantification; Mass Spectrometry; Phosphoproteome; Plant Biology; Protein Degradation; Signal Transduction; Stress response |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 28 May 2020 13:23 |
Last Modified: | 28 May 2020 13:23 |
Status: | Published online |
Publisher: | American Society for Biochemistry & Molecular Biology |
Identification Number: | 10.1074/mcp.ra119.001826 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:161274 |