Brabham, Robin, Keenan, Tessa, Husken, Annika et al. (5 more authors) (2020) Rapid sodium periodate cleavage of an unnatural amino acid enables unmasking of a highly reactive α-oxo aldehyde for protein bioconjugation. Organic and Biomolecular Chemistry. ISSN 1477-0539
Abstract
The α-oxo aldehyde is a highly reactive aldehyde for which many protein bioconjugation strategies exist. Here, we explore the genetic incorporation of a threonine-lysine dipeptide into proteins, harbouring a “masked” α-oxo aldehyde that is rapidly unveiled in four minutes. The reactive aldehyde could undergo site-specific protein modification by SPANC ligation
Metadata
Item Type: | Article |
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Authors/Creators: |
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Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Funding Information: | Funder Grant number EPSRC EP/S013741/1 |
Depositing User: | Pure (York) |
Date Deposited: | 20 May 2020 08:30 |
Last Modified: | 17 Dec 2024 00:16 |
Published Version: | https://doi.org/10.1039/d0ob00972e |
Status: | Published online |
Refereed: | Yes |
Identification Number: | 10.1039/d0ob00972e |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:161006 |
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Filename: d0ob00972e.pdf
Description: Rapid sodium periodate cleavage of an unnatural amino acid enables unmasking of a highly reactive α-oxo aldehyde for protein bioconjugation
Licence: CC-BY 2.5