Dikici, S. orcid.org/0000-0001-9933-5254, Claeyssens, F. orcid.org/0000-0002-1030-939X and MacNeil, S. orcid.org/0000-0002-9188-5769 (2020) Bioengineering vascular networks to study angiogenesis and vascularisation of physiologically relevant tissue models in vitro. ACS Biomaterials Science & Engineering, 6 (6). pp. 3513-3528. ISSN 2373-9878
Abstract
Angiogenesis assays are essential for studying aspects of neovascularisation and angiogenesis and investigating drugs that stimulate or inhibit angiogenesis. To date, there are several in vitro and in vivo angiogenesis assays that are used for studying different aspects of angiogenesis. Although in vivo assays are the most representative of native angiogenesis, they raise ethical questions, require considerable technical skills, and are expensive. In vitro assays are inexpensive and easier to perform, but the majority of them are only two-dimensional cell monolayers which lack the physiological relevance of three-dimensional structures. Thus, it is important to look for alternative platforms to study angiogenesis under more physiologically relevant conditions in vitro. Accordingly, in this study, we developed polymeric vascular networks to be used to study angiogenesis and vascularisation of a 3D human skin model in vitro. Our results showed that this platform allowed the study of more than one aspect of angiogenesis, endothelial migration and tube formation, in vitro when combined with Matrigel®. We successfully reconstructed a human skin model, as a representative of a physiologically relevant and complex structure, and assessed the suitability of the developed in vitro platform for studying endothelialisation of the tissue-engineered skin model.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 American Chemical Society. This is an open access article published under a Creative Commons Attribution (CC-BY) License, (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
Keywords: | angiogenesis; vascularisation; 3D model; angiogenesis model; tissue engineering; skin; tissue-engineered skin model |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Funding Information: | Funder Grant number MEDICAL RESEARCH COUNCIL MR/L012669/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL EP/I007695/1 ROYAL SOCIETY IE160615 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 07 May 2020 11:28 |
Last Modified: | 22 Nov 2021 11:25 |
Status: | Published |
Publisher: | American Chemical Society (ACS) |
Refereed: | Yes |
Identification Number: | 10.1021/acsbiomaterials.0c00191 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:160448 |