Kimura, A., Yamauchi, Y., Hodono, S. et al. (5 more authors) (2017) Treatment response of ethyl pyruvate in a mouse model of chronic obstructive pulmonary disease studied by hyperpolarized129Xe MRI. Magnetic Resonance in Medicine, 78 (2). pp. 721-729. ISSN 0740-3194
Abstract
Purpose The purpose of this work was to investigate disease progression and treatment response in a murine model of chronic obstructive pulmonary disease (COPD) using a preclinical hyperpolarized 129Xe (HPXe) magnetic resonance imaging (MRI) strategy.
Methods COPD phenotypes were induced in 32 mice by 10 weeks of exposure to cigarette smoke (CS) and lipopolysaccharide (LPS). Efficacy of ethyl pyruvate (EP), an anti‐inflammatory drug, was investigated by administering EP to 16 of the 32 mice after 6 weeks of CS and LPS exposure. HPXe MRI was performed to monitor changes in pulmonary function during disease progression and pharmacological therapy.
Results HPXe metrics of fractional ventilation and gas‐exchange function were significantly reduced after 6 weeks of CS and LPS exposure compared to sham‐instilled mice administered with saline (P < 0.05). After this observation, EP administration was started in 16 of the 32 mice and continued for 4 weeks. EP was found to improve HPXe MRI metrics to a similar level as in sham‐instilled mice (P < 0.01). Histological analysis showed significant alveolar tissue destruction in the COPD group, but relatively normal alveolar structure in the EP and sham‐instilled groups.
Conclusion This study demonstrates the potential efficacy of EP for COPD therapy, as assessed by a noninvasive, translatable 129Xe MRI procedure. Magn Reson Med 78:721–729, 2017. © 2016 International Society for Magnetic Resonance in Medicine
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 International Society for Magnetic Resonance in Medicine. This is an author-produced version of a paper subsequently published in Magnetic Resonance in Medicine. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | hyperpolarized 129Xe MRI; lung functional assessment; murine chronic obstructive pulmonary disease; treatment response; ethyl pyruvate |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection and Immunity (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 12 May 2020 12:57 |
Last Modified: | 13 May 2020 23:48 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/mrm.26458 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:160340 |