Komarov, D.A., Ichikawa, Y., Yamamoto, K. et al. (7 more authors) (2018) In vivo extracellular pH mapping of tumors using electron paramagnetic resonance. Analytical Chemistry, 90 (23). pp. 13938-13945. ISSN 0003-2700
Abstract
An electron paramagnetic resonance (EPR)-based method for noninvasive three-dimensional extracellular pH mapping was developed using a pH-sensitive nitroxyl radical as an exogenous paramagnetic probe. Fast projection scanning with a constant magnetic field sweep enabled the acquisition of four-dimensional (3D spatial +1D spectral) EPR images within 7.5 min. Three-dimensional maps of pH were reconstructed by processing the pH-dependent spectral information on the images. To demonstrate the proposed method of pH mapping, the progress of extracellular acidosis in tumor-bearing mouse legs was studied. Furthermore, extracellular pH mapping was used to visualize the spatial distribution of acidification in different tumor xenograft mouse models of human-derived pancreatic ductal adenocarcinoma cells. The proposed EPR-based pH mapping method enabled quantitative visualization of regional changes in extracellular pH associated with altered tumor metabolism.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 American Chemical Society. This is an author-produced version of a paper subsequently published in Analytical Chemistry. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Animals; Carcinoma, Pancreatic Ductal; Carcinoma, Squamous Cell; Electron Spin Resonance Spectroscopy; Humans; Hydrogen-Ion Concentration; Imaging, Three-Dimensional; Mice; Neoplasms, Experimental |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection and Immunity (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 11 May 2020 15:59 |
Last Modified: | 11 May 2020 15:59 |
Status: | Published |
Publisher: | American Chemical Society (ACS) |
Refereed: | Yes |
Identification Number: | 10.1021/acs.analchem.8b03328 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:160338 |