Snowden, JS orcid.org/0000-0001-7857-0634, Hurdiss, DL orcid.org/0000-0003-3834-5808, Adeyemi, OO orcid.org/0000-0002-0848-5917 et al. (3 more authors) (2020) Dynamics in the murine norovirus capsid revealed by high-resolution cryo-EM. PLOS Biology, 18 (3). e3000649. ISSN 1544-9173
Abstract
Icosahedral viral capsids must undergo conformational rearrangements to coordinate essential processes during the viral life cycle. Capturing such conformational flexibility has been technically challenging yet could be key for developing rational therapeutic agents to combat infections. Noroviruses are nonenveloped, icosahedral viruses of global importance to human health. They are a common cause of acute gastroenteritis, yet no vaccines or specific antiviral agents are available. Here, we use genetics and cryo-electron microscopy (cryo-EM) to study the high-resolution solution structures of murine norovirus as a model for human viruses. By comparing our 3 structures (at 2.9- to 3.1-Å resolution), we show that whilst there is little change to the shell domain of the capsid, the radiating protruding domains are flexible, adopting distinct states both independently and synchronously. In doing so, the capsids sample a range of conformational space, with implications for maintaining virion stability and infectivity.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 Snowden et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Cryo EM, Image Processing (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Molecular Virology (Leeds) |
Funding Information: | Funder Grant number MRC (Medical Research Council) MR/S007229/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 May 2020 14:40 |
Last Modified: | 07 May 2020 14:40 |
Status: | Published |
Publisher: | Public Library of Science |
Identification Number: | 10.1371/journal.pbio.3000649 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:159870 |
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