Lorent, Christian, Katz, Sagie, Duan, Jifu et al. (9 more authors) (2020) Shedding Light on Proton and Electron Dynamics in [FeFe] Hydrogenases. Journal of the American Chemical Society. pp. 5493-5497. ISSN 1520-5126
Abstract
[FeFe] hydrogenases are highly efficient catalysts for reversible dihydrogen evolution. H2 turnover involves different catalytic intermediates including a recently characterized hydride state of the active site (H-cluster). Applying cryogenic infrared and electron paramagnetic resonance spectroscopy to an [FeFe] model hydrogenase from Chlamydomonas reinhardtii (CrHydA1), we have discovered two new hydride intermediates and spectroscopic evidence for a bridging CO ligand in two reduced H-cluster states. Our study provides novel insights into these key intermediates, their relevance for the catalytic cycle of [FeFe] hydrogenase, and novel strategies for exploring these aspects in detail.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 American Chemical Society. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 07 Apr 2020 13:50 |
Last Modified: | 04 Mar 2025 00:06 |
Published Version: | https://doi.org/10.1021/jacs.9b13075 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1021/jacs.9b13075 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:159285 |