Herr, P. orcid.org/0000-0003-2945-966X, Boström, J., Rullman, E. et al. (6 more authors) (2020) Cell cycle profiling reveals protein oscillation, phosphorylation, and localization dynamics. Molecular & Cellular Proteomics, 19 (4). pp. 608-623. ISSN 1535-9476
Abstract
The cell cycle is a highly conserved process involving the coordinated separation of a single cell into two daughter cells. To relate transcriptional regulation across the cell cycle with oscillatory changes in protein abundance and activity, we carried out a proteome- and phospho-proteome-wide mass spectrometry profiling. We compared protein dynamics with gene transcription, revealing many transcriptionally regulated G2 mRNAs that only produce a protein shift after mitosis. Integration of CRISPR/Cas9 survivability studies further highlighted proteins essential for cell viability. Analyzing the dynamics of phosphorylation events and protein solubility dynamics over the cell cycle, we characterize predicted phospho-peptide motif distributions and predict cell cycle-dependent translocating proteins, as exemplified by the S-adenosylmethionine synthase MAT2A. Our study implicates this enzyme in translocating to the nucleus after the G1/S-checkpoint, which enables epigenetic histone methylation maintenance during DNA replication. Taken together, this data set provides a unique integrated resource with novel insights on cell cycle dynamics.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2020 Herr et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. This is an Open Access article under a CC BY license.(https://creativecommons.org/licenses/by/4.0/) |
Keywords: | Cell cycle; phosphorylation; subcellular separation; computational biology; systems biology |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 08 Apr 2020 07:11 |
Last Modified: | 16 Aug 2021 14:16 |
Status: | Published |
Publisher: | American Society for Biochemistry & Molecular Biology (ASBMB) |
Refereed: | Yes |
Identification Number: | 10.1074/mcp.ra120.001938 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:159275 |