Dardente, H., English, W.R. orcid.org/0000-0003-3024-2441, Valluru, M.K. et al. (2 more authors) (2020) Debunking the myth of the endogenous antiangiogenic Vegfaxxxb transcripts. Trends in Endocrinology & Metabolism, 31 (6). pp. 398-409. ISSN 1043-2760
Abstract
In this opinion article we critically assess evidence for the existence of a family of antiangiogenic vascular endothelial growth factor (Vegfaxxxb) transcripts, arising from the use of a phylogenetically conserved alternative distal splice site within exon 8 of the VEGFA gene. We explain that prior evidence for Vegfaxxxb transcripts in tissues rests heavily upon flawed RT-PCR methodologies, with the extensive use of 5′-tailing in primer design being the main issue. Furthermore, our analysis of large RNA-seq data sets (human and ovine) fails to identify a single Vegfaxxxb transcript. Therefore, we challenge the very existence of Vegfaxxxb transcripts, which further questions the physiological relevance of studies based on the use of ‘anti-VEGFAxxxb’ antibodies. Our analysis has implications for the proposed therapeutic use of isoform-specific anti-VEGFA strategies for treating cancer and retinopathies.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 Elsevier Ltd. This is an author produced version of a paper subsequently published in Trends in Endocrinology and Metabolism. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
Keywords: | VEGFA; transcription; RNA-seq; alternative splicing; angiogenesis; cancer; retina |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Human Metabolism (Sheffield) The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Oncology (Sheffield) |
Funding Information: | Funder Grant number WESTON PARK HOSPITAL CANCER CHARITY SG224 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 25 Feb 2020 11:12 |
Last Modified: | 10 Dec 2021 14:00 |
Status: | Published |
Publisher: | Elsevier BV |
Refereed: | Yes |
Identification Number: | 10.1016/j.tem.2020.01.014 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:157656 |