Wright, KE orcid.org/0000-0002-0387-3048, Liniker, E, Loizidou, M et al. (3 more authors) (2009) Peripheral neural cell sensitivity to mTHPC-mediated photodynamic therapy in a 3D in vitro model. British Journal of Cancer, 101 (4). pp. 658-665. ISSN 0007-0920
Abstract
Background:
The effect of photodynamic therapy (PDT) on neural cells is important when tumours are within or adjacent to the nervous system. The purpose of this study was to investigate PDT using the photosensitiser, meta-tetrahydroxyphenyl chlorin (mTHPC), on rat neurons and satellite glia, compared with human adenocarcinoma cells (MCF-7).
Methods:
Fluorescence microscopy confirmed that mTHPC was incorporated into all three cell types. Sensitivity of cells exposed to mTHPC-PDT (0–10 μg ml–1) was determined in a novel 3-dimensional collagen gel culture system. Cell death was quantified using propidium iodide and cell types were distinguished using immunocytochemistry. In some cases, neuron survival was confirmed by measuring subsequent neurite growth in monolayer culture.
Results:
MCF-7s and satellite glia were significantly more sensitive to PDT than neurons. Importantly, 4 μg ml–1 mTHPC-PDT caused no significant neuron death compared with untreated controls but was sufficient to elicit substantial cell death in the other cell types. Initially, treatment reduced neurite length; neurons then extended neurites equivalent to those of untreated controls. The protocol was validated using hypericin (0–3 μg ml–1), which caused neuron death equivalent to other cell types.
Conclusion:
Neurons in culture can survive mTHPC-PDT under conditions sufficient to kill tumour cells and other nervous system cells.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Ⓒ 2009 Cancer Research UK. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. |
Keywords: | PDT; Foscan; neuron; satellite glial cell; MCF-7 cell; 3D culture |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Geography (Leeds) > River Basin Processes & Management (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 09 Dec 2022 09:24 |
Last Modified: | 09 Dec 2022 09:24 |
Status: | Published |
Publisher: | Springer Nature |
Identification Number: | 10.1038/sj.bjc.6605197 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:157508 |
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