Ponjavic, A orcid.org/0000-0002-7561-1127, Ye, Y, Laue, E et al. (2 more authors) (2018) Sensitive light-sheet microscopy in multiwell plates using an AFM cantilever. Biomedical Optics Express, 9 (12). pp. 5863-5880. ISSN 2156-7085
Abstract
We present a sensitive inverted light sheet microscope, capable of single-molecule fluorescence imaging of cells in 96-well plates. Light sheet microscope designs are often complex and costly, requiring custom-made sample chambers that are incompatible with standard cell culture samples. To overcome this limitation, we have developed single-objective cantilever selective plane illumination microscopy (socSPIM), which introduces a light sheet through the objective lens of an inverted microscope using an AFM tip. We demonstrate the effectiveness of this setup by performing 3D imaging of nuclear pore complexes, as well as live whole-cell 3D imaging of lysosomes and super-resolution imaging of the T-cell membrane. The unique advantage offered by socSPIM is the minimal footprint of the cantilever, which allowed us to perform super-resolution reflected light-sheet microscopy by PAINT in 96-well plates, paving the way for high-throughput studies.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | ©2018. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics |
Depositing User: | Symplectic Publications |
Date Deposited: | 18 Feb 2020 12:39 |
Last Modified: | 18 Feb 2020 12:39 |
Status: | Published |
Publisher: | Optical Society of America |
Identification Number: | 10.1364/boe.9.005863 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:157210 |