Yates, Nick, Dowsett, Mark, Bentley, Phillip et al. (5 more authors) (2019) Aldehyde-mediated protein-to-surface tethering via controlled diazonium electrode functionalization using protected hydroxylamines. Langmuir. ISSN 1520-5827
Abstract
We report a diazonium electro-grafting method for the covalent modification of conducting surfaces with aldehyde-reactive hydroxylamine functionalities that facilitate the wiring of redox-active (bio)molecules to electrode surfaces. Hydroxylamine monolayer formation is achieved via a phthalimide-protection and hydrazine-deprotection strategy that overcomes the multilayer formation that typically complicates diazonium surface modification. This surface modification strategy is characterized using electrochemistry (electrochemical impedance spectroscopy and cyclic voltammetry), X-ray photoelectron spectroscopy and quartz crystal microbalance with dissipation monitoring. Thus-modified glassy carbon, boron-doped diamond and gold surfaces are all shown to ligate to small molecule aldehydes, yielding surface coverages of 150-170, 40 and 100 pmol cm-2, respectively. Bio-conjugation is demonstrated via the coupling of a dilute (50 µM) solution of periodate-oxidized horseradish peroxidase enzyme to a functionalized gold surface under bio-compatible conditions (H2O solvent, pH 4.5, 25 °C).
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 American Chemical Society. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Physics (York) The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 07 Feb 2020 09:20 |
Last Modified: | 26 Nov 2024 00:45 |
Published Version: | https://doi.org/10.1021/acs.langmuir.9b01254 |
Status: | Published online |
Refereed: | Yes |
Identification Number: | 10.1021/acs.langmuir.9b01254 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:156673 |